Terra qPCR Direct TB Green Premix is a 2X master mix that lets you perform real-time PCR (qPCR) directly on crude extracts or dirty samples (such as mouse tail and ear biopsies, or leaf cuttings). The premix contains everything you need for qPCR, including Terra PCR Direct Polymerase—a robust, non-Taq polymerase developed for optimal amplification from whole cells, crude cell lysates, tissue extracts, or purified DNA. The enzyme is perfect for amplifying DNA targets up to 2 kb, even when the GC content is greater than 70%. The premix also contains TB Green dye, as well as a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing an automatic hot start. In addition, Terra qPCR Direct TB Green Premix comes packaged with two ROX formulations—ROX Reference Dye LSR and ROX Reference Dye LMP—allowing you to normalize the fluorescence signal between reactions on instruments that are equipped with this option.
Terra qPCR Direct TB Green Premix is a 2X master mix designed specifically for real-time PCR with TB Green chemistry. In addition to TB Green dye, this mix contains Terra qPCR Direct Polymerase, a novel enzyme developed for optimal amplification from crude or dirty templates; it’s perfect for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The premix also contains a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing automatic hot start PCR.
Perform real-time PCR with crude tissue extracts or dirty samples—no template purification necessary
Easily amplify GC-rich targets
Convenient 2X master mix contains TB Green dye
Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice
Automatic hot start
Perfect for high-throughput screening applications
Perform direct real-time PCR from crude extracts and dirty samples, without having to purify your template
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.