TB Green® Premix Ex Taq™ (Tli RNase H Plus)

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TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

Overview

  • Lower Cq values as a result of fast extension rates
  • Use with broad range of amplicon sizes, even over 500 bp
  • Convenient 2X master mix is excellent for high-speed qPCR
  • High sensitivity: detects fewer than 100 copies
  • Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
  • Compatible with all commonly used real-time PCR (qPCR) instruments
  • Minimizes qPCR inhibition due to residual mRNA in input cDNA
  • Includes TB Green for intercalator-based qPCR

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