A convenient, 2X PCR master mix containing Takara Ex Taq DNA polymerase, optimized buffer, and dNTPs. Please refer to the User Manual for buffer composition. Takara Ex Taq DNA polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA.
- High-yield, high-sensitivity PCR
- Premixed PCR master mix for convenient amplification requiring minimal assembly
- Generation of PCR products having >80% cloning efficiency in T-vectors
- Ideal for high-fidelity, high-yield endpoint PCR
Enzyme performance is confirmed by successful amplification of a 20-kb lambda DNA template. PCR amplification of a single copy gene (Beta-globin) by PCR is confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb).
PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located below.