GAPDH Mouse mAb

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Overview


Product name GAPDH Mouse mAb
Catalog No. AC033
Host species Mouse
Purification method Affinity purification
Isotype IgG


Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.

Immunogen information


Immunogen Recombinant protein of human GAPDH
Sequence Email for sequence
Gene ID 2597
Swiss prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW 31kDa/36kDa
Observed MW 36KDa

 

Immunogen Recombinant protein of human GAPDH
Sequence Email for sequence
Gene ID 2597
Swiss prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW 31kDa/36kDa
Observed MW 36KDa

 

Applications

 

Reactivity Human, Mouse, Rat
Tested applications WBIHCIF
Recommended dilution WB 1:50000 - 1:200000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Antibody Dilute Procedure Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, HepG2, 293T, Mouse liver, Mouse kidney, Mouse spleen, Rat liver, Rat kidney
Cellular location Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Schisandra chinensis)

IP(Mus musculus)

IF(Homo sapiens,Mus musculus)

DB(Homo sapiens)