|Product name||GAPDH Mouse mAb|
|Purification method||Affinity purification|
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
|Immunogen||Recombinant protein of human GAPDH|
|Sequence||Email for sequence|
|Synonyms||G3PD; GAPD; HEL-S-162eP; GAPDH|
|Reactivity||Human, Mouse, Rat|
|Recommended dilution||WB 1:50000 - 1:200000
IHC 1:50 - 1:200
IF 1:50 - 1:200
|Antibody Dilute Procedure||Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence|
|Positive samples||HeLa, HepG2, 293T, Mouse liver, Mouse kidney, Mouse spleen, Rat liver, Rat kidney|
|Cellular location||Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region|
WB(Homo sapiens, Mus musculus, Rattus norvegicus, Schisandra chinensis)
IF(Homo sapiens,Mus musculus)